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A strategy for annotating the human milk glycome.

Author: Ninonuevo MR, Park Y, Hongfeng Y, Zhang J, Ward RE and Clowers BH. | Journal: Journal of Agriculture and Food Chemistry

Volume: 54
Issue: 20
Background:Oligosaccharides in human milk represent a group of bioactive molecules that are abundant and diverse component of human milk, even though they have no direct nutritive value to the infant. A recent hypothesis proposes that they could be substrates for the development of the intestinal microflora and the mucosal immune system. The inability to determine the exact composition of these oligosaccharides limits research and the ability to understand their biological functions.
​​​​​​​ Methods:Oligosaccharides isolated from the lipids and proteins of individual human milk samples were analyzedby a combination of techniques including microchip liquid chromatography mass spectrometry (HPLCChip/MS) and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FT ICR MS). Accurate mass measurements obtained using an orthogonal time-of-flight (o-TOF) mass spectrometry provided oligosaccharide composition for approximately 200 individual molecular species. ​​​​​​​
​​​​​​​ Results:Comparison of HPLC-Chip/MS profiles from five different women revealed variations in milk oligosaccharide compositions. Read More

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